Premium
Evaluation of the authenticity of haloarchaeal strains by random‐amplified polymorphic DNA
Author(s) -
MartínezMurcia A.J.,
Boán I.F.,
RodríguezValera F.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01018.x
Subject(s) - rapd , strain (injury) , biology , microbiology and biotechnology , dna , genetics , bacteria , genetic diversity , population , demography , sociology , anatomy
PCR analysis was performed on several archaeal species using 11 different 10‐mer oligonucleotides of arbitrary sequence. Duplicates of the type strain of Halococcus morrhuae (ATCC 17082 and CCM 537) showed markedly different RAPD profiles. Moreover, RAPD patterns from the type strain of Haloferax denitrificans ATCC 35960 and the strain ATCC 49116 deposited as ‘Haloferax larsenii’ , were identical. This method represents an invaluable instrument in microbial screening and can clarify problems with strain identities.