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Escherichia coli JA221 can suppress the UAG stop signal
Author(s) -
Lacadena J.,
Pozo A. Martinez,
Mancheño J.M.,
Gasset M.,
Oñaderra M.,
Gavilanes J.G.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01015.x
Subject(s) - escherichia coli , strain (injury) , stop codon , recombinant dna , plasmid , signal peptide , biology , chemistry , microbiology and biotechnology , genetics , gene , anatomy
The Escherichia coli strain JA221 can suppress the UAG stop codon, although the existence of an amber suppressor tRNA has not previously been described for this strain. When using a plasmid to express α‐sarcin, which has TAG as its stop signal, two proteins were obtained: a smaller protein corresponding in size to that of the expected protein, and a larger protein, which could be accounted for by the presence of a second stop codon (TGA) 18 base pairs downstream of the original. This feature of strain JA221 must therefore be considered when using this strain as a host for the production of recombinant proteins.