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Comparative study of substrates of fungal laccase
Author(s) -
Lonergan G.,
Baker W.L.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb01000.x
Subject(s) - laccase , abts , hydrogen peroxide , catalase , peroxidase , chemistry , enzyme , substrate (aquarium) , biochemistry , peroxide , biology , organic chemistry , antioxidant , ecology , dpph
Coriolus versicolor, Pycnoporus cinnabarinus and Pycnoporus coccineus were grown under conditions to produce extracellular laccase. Prior to estimating enzyme activity, culture fluids were pretreated with catalase to destroy hydrogen peroxide and hence minimize peroxidase activity which might interfere with laccase determinations. Similar trends in enzyme assay were shown when colour reagents contained either syringaldazine or 3‐dimethylaminobenzoic acid plus 3‐methyl‐2‐benzothiazolone hydrasone as laccase substrates. Use of 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline)‐6‐sulphonate (ABTS) as laccase substrate showed a different trend which was attributed to peroxidatic activity of the catalase using hydrogen peroxide generated by fungal oxidases. Peroxidatic activity was not observed with the other substrates.