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A rapid protocol for isolation of RNA from mycobacteria
Author(s) -
Rajagopalan M.,
Boggaram V.,
Madiraju M.V.V.S.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb00995.x
Subject(s) - rna , rna extraction , ethidium bromide , biology , microbiology and biotechnology , centrifugation , transcription (linguistics) , dna , gene , biochemistry , linguistics , philosophy
Isolation of total cellular RNA from members of mycobacteria has been a labour‐intensive task involving large volumes of cells, multiple extractions of cell lysates with phenol–chloroform followed by caesium chloride centrifugation. A simple and rapid procedure is reported for isolation of RNA from mycobacteria using as few as 1 times 10 7 cells. The RNA thus isolated when analysed on ethidium bromide gels contained 16S and 23S RNA as major species. Further, the RNA was used for amplification of an internal segment of hsp65 gene by reverse transcription followed by PCR.