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A rapid general method for the identification of PCR products using a fibre‐optic biosensor and its application to the detection of Listeria
Author(s) -
Strachan N.J.C.,
Gray D.I.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb00993.x
Subject(s) - polymerase chain reaction , listeria , biosensor , listeria monocytogenes , agarose , agarose gel electrophoresis , microbiology and biotechnology , biology , gel electrophoresis , chromatography , fluorescein , chemistry , gene , bacteria , fluorescence , biochemistry , genetics , optics , physics
A 200‐mer fragment of the fla A gene from Listeria monocytogenes was amplified using the polymerase chain reaction (PCR) incorporating biotin‐ and fluorescein amadite (FAM‐)labelled primers. Methods are described for isolating the single stranded FAM‐labelled 200‐mer. A central portion of this 200‐mer was successfully hybridized onto a complementary sequence coated onto a fibre optic biosensor. Advantages in the specificity and speed of this approach compared to standard agarose gel electrophoresis and probing methods are discussed.