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Listeriolysin O secretion by Listeria monocytogenes in the presence of cysteine and sorbate
Author(s) -
Kouassl Yao,
Shelef Leora A.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb00449.x
Subject(s) - listeriolysin o , listeria monocytogenes , potassium sorbate , cysteine , microbiology and biotechnology , secretion , hemolysin , biochemistry , chemistry , biology , listeria , enzyme , bacteria , virulence , genetics , sugar , gene
The influence of cysteine or cysteine‐HCl and their combination with potassium sorbate on growth of Listeria monocytogenes and listeriolysin O (LLO) secretion, and on activation of LLO in the haemolysin assay system was studied. Both cysteine and cysteine‐HCl (10 and 20 mmol 1 ‐1 ) enhanced LLO secretion in tryptic soy broth supplemented with yeast extract during 24 h incubation at 35°C. While sorbate did not affect growth, it suppressed both LLO secretion and LLO activation by cysteine in the haemolysin activity assay. These findings provide further evidence that sulphydryl‐containing enzymes are implicated in the mechanism of microbial inhibition of sorbate. Addition of sorbate to foods has the potential of inactivating listeriolysin and other thiol‐dependent toxins.