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Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler
Author(s) -
Black S.F.,
Gray D.I.,
Fenlon D.R.,
Kroll R.G.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb00424.x
Subject(s) - listeria monocytogenes , rapd , serotype , listeria , biology , primer (cosmetics) , microbiology and biotechnology , capillary electrophoresis , dna profiling , polymerase chain reaction , dna , bacteria , genetics , chemistry , gene , population , genetic diversity , demography , organic chemistry , sociology
Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10‐mer primer (5′‐ACCGCCTGCT‐3′) which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serotypes were produced from serotypes 1a, 2, 3a, 4ab, 4a and 4c but serotypes, 1/2a, 4b, 4d and 7 had similar profiles.