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Sequential heat‐curing of Tn 5 ‐Mob‐ sac labelled plasmids from Rhizobium to obtain derivatives with various combinations of plasmids and no plasmid
Author(s) -
MoënneLoccoz Y.,
Baldani J.I.,
Weaver R.W.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb00420.x
Subject(s) - plasmid , rhizobium leguminosarum , rhizobium , rhizobia , biology , rhizobiaceae , kanamycin , microbiology and biotechnology , bacteria , genetics , gene , antibiotics , symbiosis
All four plasmids from Rhizobium leguminosarum bv. trifolii W14‐2 were sequentially labelled with Tn 5 ‐Mob‐ sac , which codes for resistance to kanamycin and sensitivity to sucrose, and cured by exposing rhizobia to supra‐optimal temperatures. This is the first report where a plasmid‐less derivative of Rhizobium was obtained. No relationship was found between plasmid size and elimination. The efficiency of Hynes' selection system decreased when used to cure plasmids from rhizobial derivatives already lacking one plasmid or more. The authors' results suggest that previous reports of failure in curing Tn 5 ‐Mob‐ sac labelled plasmids may only be due to an insufficient number of colonies being screened.