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Denitrification by a mixture of bacterial strains derived from an upflow sludge blanket reactor, following entrapment in sol‐gel glass
Author(s) -
Armon R.,
Starosvetzky J.,
Green M.
Publication year - 1995
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1995.tb00399.x
Subject(s) - blanket , denitrification , environmental science , chemistry , geography , nitrogen , archaeology , organic chemistry
Whole cell extracts of a bacterial mixture grown in an upflow sludge blanket reactor used for drinking water denitrification, were successfully entrapped into sol‐gel glass, and afterwards air dried. The sol‐gel complex was able to reduce sodium nitrate and nitrite solutions. Compared with free whole cell extract, the sol‐gel complex required twice as much time for nitrate and nitrite reduction. This study is the first successful attempt to entrap denitrifier reductases for experimental denitrification into sol‐gel glass. Denitrifying enzymes entrapped into sol‐gel glass matrix, can be used several times without significant loss in specificity, but gradual loss in reactivity occurs, probably due to electron donor depletion.