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A stable L‐form of the fish pathogen Aeromonas salmonicida
Author(s) -
Gibb A.,
Austin B.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00985.x
Subject(s) - fish <actinopterygii> , aeromonas salmonicida , library science , biological sciences , fishery , biology , microbiology and biotechnology , computer science
A stable L‐form of Aeromonas salmonicida , which resulted from induction with benzylpenicillin, grew on brain heart infusion agar at 0–5°C. The L‐form was stored successfully for 10 months in phosphate‐buffered saline supplemented with 150 g l ‐1 glycerol at ‐70°C. Reversion of the L‐form to parental‐type walled cells was achieved by transfer to brain heart infusion broth with incubation at 25°C.