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Pulsed‐field gel electrophoretic identification of Salmonella enterica serovar Typhimurium live vaccine strain Zoosaloral H
Author(s) -
Schwarz S.,
Liebisch Babett
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00984.x
Subject(s) - serotype , salmonella enterica , salmonella , strain (injury) , microbiology and biotechnology , biology , virology , bacteria , genetics , anatomy
Salmonella enterica subsp. enterica serovar Typhimurium ( Salm. Typhimurium) live vaccine strain Zoosaloral H was characterized by pulsed‐field gel electrophoresis (PFGE). Each of the two suitable restriction enzymes, Xba I and Spe I, produced a unique restriction fragment pattern for this live vaccine strain which was not shared by field isolates of the same serovar. The characteristic fragment pattern proved to be stable during a 22 month observation period and was also not altered after animal passage of the vaccine strains. Thus PFGE analysis proved to be a helpful tool in the identification of Salm. Typhimurium live vaccine strain Zoosaloral H and its differentiation from wild‐type isolates of the same serovar.