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Isolation and selection of an L‐aminoacylase‐producing bacterium, Pseudomonas sp. BA2
Author(s) -
Santoyo A. Bódalo,
Rodríguez J. Bastida,
Iniesta F. Marín,
Gómez E. Gómez,
Teruel M.L. Asanza,
Rojo I. Alcaraz
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00982.x
Subject(s) - pseudomonas fluorescens , bacteria , pseudomonas , pseudomonadaceae , strain (injury) , microbiology and biotechnology , biology , pseudomonadales , enzyme , biochemistry , genetics , anatomy
The enrichment culture method was used to detect and isolate L‐aminoacylase‐producing bacteria from soil, using N ‐acetyl‐L‐alanine as inducer and substrate. Isolated bacterial strains were screened for growth and enzyme activity. Strain BA2 displayed both the highest intracellular L‐aminoacylase activity and the most profuse growth. Furthermore, BA2 cells did not show any D‐aminoacylase activity. This strain was an obligately aerobic rod‐shaped bacterium and stained Gram‐negative, and was therefore identified as Pseudomonas. Its morphological and biochemical characteristics corresponded to those of Pseudomonas fluorescens biovar I.