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Bioluminescent Salmonella typhimurium provides a rapid assay for measuring the efficacy of freeze‐drying suspension media
Author(s) -
Russell Julie E.,
Perry S.F.,
Roberts Diane
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00971.x
Subject(s) - bioluminescence , salmonella , skimmed milk , gelatin , suspension (topology) , microbiology and biotechnology , chromatography , strain (injury) , chemistry , inositol , suspension culture , bacteria , freeze drying , food science , biology , biochemistry , cell culture , genetics , receptor , mathematics , anatomy , homotopy , pure mathematics
A strain of Salmonella typhimurium , transformed to a bioluminescent phenotype, was used to compare three freeze‐drying suspension media: inositol serum broth with and without added gelatin and sterile skimmed milk. Recovery and growth studies performed by measuring changes in bioluminescence demonstrated that of the three media tested, the routinely used inositol serum broth was the most effective freeze‐drying suspension medium.