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Rapid identification of pathogenic neisserias using the Identicult‐Neisseria test
Author(s) -
Moyes A.,
Chronas G.,
Young H.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00951.x
Subject(s) - neisseria , microbiology and biotechnology , biology , identification (biology) , neisseria gonorrhoeae , bacteria , genetics , ecology
A rapid enzymatic method using chromogenic substrates for the rapid identification of pathogenic neisseria (Identicult‐Neisseria, Scott Laboratories Inc., CA, USA) was tested in parallel with the rapid carbohydrate utilization test (RCUT) and the Phadebact Monoclonal GC Test against 198 consecutive clinical isolates of oxidase‐positive Gram‐negative diplococci (118 Neisseria gonorrhoeae , 76 N. meningitidis and four N. lactamica ). On initial testing the Identicult‐Neisseria gave a 95% overall concordance (97.5% N. gonorrhoeae , 90.8% N. meningitidis ). with the RCUT and Phadebact tests; the corresponding figures after repeat testing were 98% overall concordance (98.3% N. gonorrhoeae , 97.4% N. meningitidis ). Two of the three strains of N. gonorrhoeae mis‐identified as N. meningitidis on primary testing were also mis‐identified on repeat testing. Seven strains of N. meningitidis were mis‐identified on initial testing (six as Moraxella catarrhalis and one as N. lactamica ) and two on repeat testing (both as Mor. catarrhalis ). We conclude that the Identicult‐Neisseria is not sufficiently reliable for the culture confirmation of gonococci and meningococci.