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Evaluation of Bacillus subtilis strain IS53 for the production of Bacillus anthracis protective antigen
Author(s) -
Baillie L.W.J.,
Johnson M.,
Manchee R.J.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00949.x
Subject(s) - bacillus anthracis , bacillus subtilis , strain (injury) , microbiology and biotechnology , proteases , antigen , plasmid , bacillaceae , biology , bacillales , extracellular , toxin , lysis , bacteria , chemistry , biochemistry , enzyme , gene , immunology , genetics , anatomy
An asporogenous strain of Bacillus subtilis , IS53, transformed with plasmid pPA102, produces the protective antigen (PA) component of the tripartite toxin of B. anthracis . Addition of yeast extract was required to support growth and PA production in all the media examined. Protective antigen expression was down‐regulated during exponential growth and extracellular proteases caused marked degradation of the mature protein.