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Cell surface charge and initial attachment characteristics of rough strains of Listeria monocytogenes
Author(s) -
Dickson J.S.,
Siragusa G.R.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00941.x
Subject(s) - listeria monocytogenes , surface charge , cell , listeria , charge (physics) , chemistry , biophysics , bacteria , food science , chromatography , biology , microbiology and biotechnology , biochemistry , physics , genetics , quantum mechanics
Mention of trade name, proprietary product or specific equipment is necessary to report factually on available data: however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval to the exclusion of others that may be suitable. The relative negative surface charge and hydrophobicity of four bacterial strains were evaluated by gravity flow and spin column methods. There was no significant difference between the two methods, indicating that spin column chromatography is an acceptable alternative method of determining cell surface charge or hydrophobicity. Six strains of Listeria monocytogenes which exhibited rough colony appearance were evaluated for surface charge and hydrophobicity and their ability to contaminate beef muscle tissue. With one exception, all of the rough strains exhibited greater net negative surface charge and reduced ability to contaminate beef during the initial stages of attachment. Since greater net negative cell surface charge has been positively correlated to attachment to beef tissue surfaces, the reduction in attachment exhibited by the rough strains was interpreted as an indication that attachment to beef tissue cannot be solely explained by cell surface charge.