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Characterization of phe B gene encoding catechol 2,3‐dioxygenase
Author(s) -
Nishihara T.,
Yamada T.,
Takano K.,
Osada S.,
Nishikawa J.,
Imagawa M.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00938.x
Subject(s) - library science , chinese academy of sciences , political science , computer science , china , law
The DNA sequence of the phe B gene isolated from a chromosome of the phenol degrading bacterium Pseudomonas putida BH is identical to that of the dmp B gene from the phenol degradative plasmid, pVI150. Catechol 2,3‐dioxygenase encoded by phe B showed similar substrate specificity to that of xyl E. However, Phe B has much smaller K m values than Xyl E, indicating that Phe B is useful for treatment of low concentrations of catechol derivatives in waste water.