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Identification of Carnobacterium spp. and Leuconostoc spp. in meat by genus‐specific 16S rRNA probes
Author(s) -
Nissen Hilde,
Holck A.,
Dainty R. H.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00933.x
Subject(s) - leuconostoc , biology , 16s ribosomal rna , nucleic acid , bacteria , oligonucleotide , ribosomal rna , microbiology and biotechnology , lactic acid , biochemistry , dna , genetics , gene
Oligonucleotide probes specific for Carnobacterium and Leuconostoc species were constructed from the variable regions of 16S rRNA obtained from the literature and sequence data bases. The probes were hybridized with crude nucleic acid extract from 32 type strains of lactic acid bacteria (LAB) commonly found on meat. Two of the probes hybridized only to the four Carnobacterium species whereas the other two hybridized only to five of the six Leuconostoc species tested. The probes were also hybridized with nucleic acids from unknown strains of LAB. The identification was consistent with the results of biochemical tests used to characterize the two genera.