Premium
Influence of acidification and type of acidulant of the recovery medium on Bacillus stearothermophilus spore counts
Author(s) -
Fernandez P.S.,
Gomez F.J.,
Ocio M.J.,
Sanchez T.,
Rodrigo M.,
Martinez A.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00928.x
Subject(s) - spore , citric acid , bacillales , food science , bacillus (shape) , chemistry , bacillaceae , bacteria , microbiology and biotechnology , biology , bacillus subtilis , genetics
Unheated and heated (1 min at 121°C) Bacillus stearothermophilus spores were grown in non‐acidified (pH 7) and acidified recovery medium at different pH levels (6.7, 6.5, 6.2 and 5.9) using citric acid or glucono‐δ‐lactone (GDL) as acidulants. It was observed that the lower the pH, the lower the proportion of micro‐organisms which recovered. The level of recovery was not the same for both acidulants, meaning that at the same pH level citric acid showed a greater inhibitory effect that glucono‐δ‐lactone. Heat treatment sensitized spores to pH effect. A linear relationship of the behaviour of spores with each acidulant is proposed.