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CCD‐monitoring of bioluminescence during the induction of the cell wall‐deficient, L‐form state of a genetically modified strain of Pseudomonas syringae pv. phaseolicola
Author(s) -
Waterhouse Rosemary N.,
Glover L. Anne
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00912.x
Subject(s) - pseudomonas syringae , bioluminescence , pseudomonadaceae , strain (injury) , pseudomonadales , microbiology and biotechnology , biology , bacteria , pseudomonas aeruginosa , pathogen , genetics , biochemistry , anatomy
Bioluminescence from developing L‐form colonies of the plant pathogen, Pseudomonas syringae pv. phaseolicola , was monitored using the enhanced light‐detecting capabilities of a charge‐coupled device. During L‐form induction, the bacteria entered a prolonged period during which the level of light output and hence metabolic activity, was very low. A relatively small number of highly bioluminescent L‐form colonies were then observed to develop against a background of non‐bioluminescent bacteria. When these colonies were sub‐cultured and examined microscopically, typical L‐form morphology was observed and continued high bioluminescence was detectable from derived colonies.