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Comparison of combinations of diluents and media for enumerating Zygosaccharomyces rouxii in intermediate water activity foods
Author(s) -
AbdulRaouf U.M.,
Hwang C.A.,
Beuchat L.R.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00896.x
Subject(s) - diluent , food science , chemistry , microbiology and biotechnology , biology , organic chemistry
Combinations of five diluents (0.1% peptone, 40 and 50% glucose, and 18 and 26% glycerol) and three enumeration media (tryptone glucose yeast extract, dichloran 18% glycerol and malt extract yeast extract 50% glucose (MY50G) agars) were evaluated for recovering a xerotolerant yeast, Zygosaccharomyces rouxii , from foods with intermediate water activity ( a w ). Combinations of 40% ( a w , 0.936) or 50% ( a w 0.898) glucose diluent and MY50G agar ( a w 0.890) were superior in recovering highest populations. The type of solute in the diluent, as well as a reduced a w , influences efficiency of recovering viable cells.