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β‐Amylase from Clostridium thermocellum SS8 ‐ a thermophilic, anaerobic, cellulolytic bacterium
Author(s) -
Swamy M.V.,
Ram M. Sai,
Seenayya G.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00875.x
Subject(s) - clostridium thermocellum , maltose , thermophile , amylase , starch , enzyme , clostridium , chemistry , biochemistry , food science , bacteria , biology , cellulase , genetics
The extracellular amylase produced by Clostridium thermocellum strain SS8 on starch was characterized as a β‐amylase based on blue value reduction test and the production of maltose from starch. The enzyme had a temperature and pH optima of 60°C and 6.0, respectively. Of the metal ions tested, Ca 2 + and Mg 2 + had little effect on enzyme activity, but their presence increased its thermal stability. Ca 2 + displayed a higher stabilizing effect and at 10 mmol 1 ‐1 Ca 2 + , the enzyme retained 86% activity even after exposure at 70°C for 30 min. The amylase was induced on starch or maltose but was repressed strongly by glucose.