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Rapid isolation and purification of lactococcal bacteriophage DNA without the use of caesium chloride gradients
Author(s) -
Brown J.C.S.,
Ward L.J.H.,
Davey G.P.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00872.x
Subject(s) - lactococcus lactis , bacteriophage , chromatography , lysis , centrifugation , dna , chemistry , restriction enzyme , elutriation , ecori , sodium , ultracentrifuge , lactococcus , bacteria , polyethylene glycol , precipitation , microbiology and biotechnology , biology , biochemistry , escherichia coli , lactic acid , genetics , gene , physics , organic chemistry , meteorology
Intact bacteriophage of Lactococcus lactis were recovered from small volumes of lysate by centrifugation at 15000 g without precipitation with polyethylene glycol and sodium chloride, or ultracentrifugation in a caesium chloride gradient. DNA was then extracted and purified by standard protocols. This DNA was readily digested with restriction endonucleases and used successfully in hybridization experiments.