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Survival and viability of Vibrio vulnificus in seawater monitored by flow cytometry
Author(s) -
Firth J.R.,
Diaper J.P.,
Edwards C.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00866.x
Subject(s) - flow cytometry , vibrio vulnificus , incubation , seawater , biology , microbiology and biotechnology , viable but nonculturable , vibrionaceae , vibrio , bacteria , cell survival , apoptosis , ecology , biochemistry , genetics
Survival and viability of Vibrio vulnificus in seawater incubated at 4°C was analysed by flow cytometry and with a fluorescent dye that stained only living cells. Although evidence was obtained that a proportion of the cells became non‐culturable, resuscitation experiments suggested that this was a transient state leading to cell death and that raising the incubation temperature from 4° to 20°C allowed numbers to increase by growth and division rather than by conversion of dormant cells to active culturable forms.