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Characterization of Bacillus licheniformis with the RAPD technique (randomly amplified polymorphic DNA)
Author(s) -
Stephan R.,
Schraft H.,
Untermann F.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00864.x
Subject(s) - rapd , bacillus licheniformis , dna , biology , bacillaceae , microbiology and biotechnology , genetics , bacillales , bacteria , bacillus subtilis , genetic diversity , medicine , population , environmental health
RAPD technique (randomly amplified polymorphic DNA) was used for epidemiological subtyping of Bacillus licheniformis and other Bacillus spp. Within 46 isolates of B. licheniformis , up to 10 strain types could be determined when two 10‐mer primers were used. RAPD patterns, which were found in eight further strains of Bacillus spp., clearly differed from those of B. licheniformis. Thus RAPD technique proved to be a promising tool for characterization of Bacillus spp.