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Molecular differentiation of two races of Fusarium oxysporum special form cubense
Author(s) -
Thomas V.,
Rutherford M.A.,
Bridge P.D.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00844.x
Subject(s) - fusarium oxysporum f.sp. cubense , fusarium oxysporum , biology , microbiology and biotechnology , botany , fusarium wilt
Total cellular DNA was extracted from 28 isolates of Fusarium oxysporum from vascular wilt of banana. The DNA samples were digested with the restriction endonuclease Hae III, and separated in agarose gels. Two characteristic banding patterns were seen in ethidium bromide stained gels. The two patterns correlated to strains believed to be of pathogenic races 1 and 4. A minor variation to these was detected with isolates of race 1 from Tanzania, which gave a typical race 1 band pattern, with an additional band of approx. 10 kb.