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Evaluation of Rambach agar for the differentiation of Salmonella species from other Enterobacteriaceae
Author(s) -
Garrick R.C.,
Smith A.D.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00842.x
Subject(s) - enterobacteriaceae , salmonella , agar , library science , biology , microbiology and biotechnology , escherichia coli , computer science , bacteria , genetics , gene
Rambach agar (Merck) was evaluated for its reliability as a selective diagnostic medium for the differentiation of Salmonella species from other Enterobacteriaceae. Twenty‐five Salmonella strains were cultured on each of three agar media, Rambach (RAM), xylose lysine desoxycholate (XLD) and bismuth sulphite (BSA). Typical, easily interpreted reactions and colony morphologies were achieved for 23 strains on RAM and BSA and 17 on XLD. Of 135 other Enterobacteriaceae cultured on RAM, 134 gave characteristics which differentiated them from Salmonella . One strain which looked like Salmonella was identified as Citrobacter freundii . Rambach agar has potential as a supplementary agar in testing foods for Salmonella , but as with other selective diagnostic agars, it has limitations.