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Identification of the Listeria monocytogenes virulence factors involved in the CAMP reaction
Author(s) -
McKellar R.C.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00809.x
Subject(s) - listeria monocytogenes , listeriolysin o , virulence , microbiology and biotechnology , biology , virulence factor , staphylococcus aureus , bacteria , agar , agar plate , listeria , biochemistry , gene , genetics
There is a need to identify the virulence factors involved in the synergistic lysis of erythrocytes (CAMP reaction) by Listeria monocytogenes and either Staphylococcus aureus or Corynebacterium equi , in order to assess the relationship between the CAMP reaction and virulence of L. monocytogenes . The ability of various L. monocytogenes mutants to secrete listeriolysin O and phospholipases, and to produce lysis of sheep blood agar was determined. The results suggest that the CAMP reaction with Coryne. equi involves listeriolysin O and Coryne. equi cholesterol oxidase, and that the reaction with Staph. aureus involves either of the phospholipases C produced by L. monocytogenes . A modified CAMP test, which incorporates cholesterol oxidase into sheep blood agar, is proposed for the rapid (4–6 h) identification of L. monocytogenes.