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Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction
Author(s) -
Powell H.A.,
Gooding C.M.,
Garrett S.D.,
Lund B.M.,
Mckee R.A.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00802.x
Subject(s) - listeria monocytogenes , polymerase chain reaction , bovine serum albumin , proteinase k , plasmin , microbiology and biotechnology , bovine milk , chemistry , biology , bacteria , enzyme , food science , biochemistry , gene , genetics
The direct detection, using the polymerase chain reaction (PCR), of Listeria monocytogenes added to cows' milk was inhibited at some milk concentrations. This inhibitor was moderately heat‐stable. Inhibition could be prevented by the addition of Bovine Serum Albumin (BSA) or proteinase inhibitors to the PCR and the evidence suggests that the inhibitor was plasmin.