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A hypercellulolytic mutant of Fusarium oxysporum
Author(s) -
Kuhad R. Chander,
Kumar M.,
Singh A.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00486.x
Subject(s) - cellulase , fusarium oxysporum , mutant , mutagenesis , extracellular , microbiology and biotechnology , hydrolysis , enzyme , biochemistry , chemistry , pectinase , protoplast , biology , botany , gene
Multiple mutagenesis of Fusarium oxysporum DSM 841 resulted in enhanced yields of cellulases. The hypercellulolytic mutant (NTG‐19) secretes high levels of extracellular cellulases on different cellulosic substrates. Addition of surfactant, Tween‐80, further increased enzyme secretion by about 30%. The results on hydrolysis of wheat straw by parent strain, DSM 841 and mutant NTG‐19 cellulases also revealed a significant improvement in the hydrolytic potential of the cellulolytic enzymes from the mutant NTG‐19.