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Expression of Lactobacillus casei ATCC 393 β‐galactosidase encoded by plasmid pLZ15 in Lactococcus lactis CNRZ 1123
Author(s) -
Hemme D.,
Gaier W.,
Winters D.A.,
Foucaud Catherine,
Vogel R.F.
Publication year - 1994
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1994.tb00471.x
Subject(s) - lactococcus lactis , lactobacillus casei , plasmid , electroporation , streptococcaceae , biology , microbiology and biotechnology , lactose , enzyme , lactococcus , lactobacillaceae , lactobacillus , bacteria , gene , biochemistry , lactic acid , genetics , antibiotics
Lactococcus lactis subsp. lactis CNRZ 1123, a Lac ‐ derivative of CNRZ 1122 was transformed by electroporation with the Lactobacillus casei ATCC 393 plasmid pLZ15, which bears a β‐galactosidase gene. The transformants expressed a constitutive β‐galactosidase activity at a higher level than in Lact. casei , and in the cell‐free extract two additional protein bands were detected by SDS‐PAGE which could correspond to lactose metabolism enzymes. Both plasmid and β‐gal activity were stable in Lactococcus after 100 generations in glucose‐containing medium.