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Sbv I restriction endonuclease from Streptococcus bovis
Author(s) -
Vanat I.,
Pristaš P.,
Kutejová E.,
Jüdová J.,
Godány A.,
Javorský P.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01471.x
Subject(s) - slovak , library science , classics , humanities , art , czech , philosophy , computer science , linguistics
Restriction endonuclease Sbv I, an isoschizomer of Hae III, has been isolated from rumen amylolytic bacterium Streptococcus bovis II/1. Sbv I was purified from cell extract by phosphocellulose chromatography and heparin‐Sepharose chromatography. The recognition sequence of Sbv I was identified by digestion of pBR322, pUC9 and Λ‐DNA and comparing the cleavage patterns obtained with computer‐derived data. Sbv I recognizes the 4‐bp palindrome, 5′‐GGCC‐3′ and cleaves DNA after the second G in the sequence, producing blunt ends.