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Rapid preparation of DNA from phytopathogenic mycoplasma‐like organisms for polymerase chain reaction analysis
Author(s) -
Firrao G.,
Locci R.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01466.x
Subject(s) - polymerase chain reaction , biology , humanities , dna , microbiology and biotechnology , art , gene , genetics
A simple procedure for the processing of MLO (mycoplasma‐like organisms)‐infected plant samples for PCR analysis is described. It is based on differential centrifugation and removal of enzyme inhibiting agents with the aid of a resin. Results of the amplifications are comparable with those achieved with more complex DNA extraction procedures. The method does not require any extraction with organic solvents or alcoholic precipitation of nucleic acids.