Premium
PCR for the detection and typing of campylobacters
Author(s) -
Birkenhead D.,
Hawkey P. M.,
Heritage J.,
GascoyneBinzi Deborah M.,
Kite P.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01455.x
Subject(s) - typing , campylobacter , biology , campylobacter jejuni , campylobacter coli , restriction fragment length polymorphism , microbiology and biotechnology , gene , polymerase chain reaction , genetics , bacteria
The flaA gene of Campylobacter sp. was amplified using PCR. Primers were chosen which amplified 1.3 kb of the flaA gene in Camp. jejuni and Camp. coli. ‘Campylobacter upsaliensis’ amplimer was approximately 1.7 kb in size and was easily distinguishable. Other species of campylobacter failed to yield amplimer. The amplimer was digested with Alu 1 which demonstrated considerable restriction fragment length polymorphism and should allow the development of a rapid novel typing scheme which does not rely on previous culture of campylobacter strains.