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Restriction analysis of an amplified polygalacturonase gene fragment differentiates strains of the phytopathogenic bacterium Pseudomonas solanacearum
Author(s) -
Gillings M.,
Fahy P.,
Davies C.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01432.x
Subject(s) - biology , polymerase chain reaction , gene , pseudomonas , restriction enzyme , microbiology and biotechnology , pectinase , bacteria , pseudomonadaceae , genetics , enzyme , biochemistry
Amplification of a polygalacturonase gene fragment using the polymerase chain reaction (PCR) formed a rapid, sensitive and portable method for detecting and differentiating strains of Pseudomonas solanacearum , a taxonomically complex bacterial species. Primers 5'CAG CAG AAC CCG CGC CTG ATC CAG 3' and 5'ATC GGA CTT GAT GCG CAG GCC GTT 3' were used to amplify a 504 base pair polygalacturonase gene fragment from 57 Ps. solanacearum isolates. Digestion of these products with Hae III defined groups which reflected the known genetic divisions within the species.