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The nucleotide sequence for the replication region of pVS40, a lactococcal food grade cloning vector
Author(s) -
Wright Attevon,
Räty Kaj
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01427.x
Subject(s) - biology , plasmid , genetics , origin of replication , ecori , nucleic acid sequence , cloning vector , coding region , microbiology and biotechnology , vector (molecular biology) , gene , recombinant dna
The replication region of a limited host range lactococcal vector, pVS40, was located within a 2359 bp Eco RI‐ Cla I fragment. Within this fragment a sequence for a 1197 bp reading frame coding for a 46 826 Da protein together with a putative ribosomal binding site and the ‐10 and ‐35 promoter regions could be detected. Immediately upstream from the promoter were three complete and one nearly complete successive direct repeats (TATAGCGTATGCTGTG), suggesting an origin of replication. The protein has considerable homologies to certain lactococcal plasmid replication proteins.