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A medium for enumeration of bacteria in foods containing swarming Bacillus spp.
Author(s) -
Thampuran Nirmala,
Surendran P. K.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01418.x
Subject(s) - enumeration , agar , bacteria , swarming (honey bee) , microbiology and biotechnology , food science , nutrient agar , bacillus (shape) , ethylenediaminetetraacetic acid , bacillales , biology , agar plate , sodium , chemistry , bacillus subtilis , genetics , mathematics , combinatorics , organic chemistry , chelation
A new medium containing sodium tripolyphosphate, ethylenediaminetetraacetic acid disodium salt, dihydrate, sodium desoxycholate, supplemented with minerals and nutrient sources was developed, which was very effective in inhibiting swarming of Bacillus spp. This medium polyphosphate‐EDTA‐desoxycholate agar (PEDA) was compared with tryptone glucose extract agar (Oxoid) for determining the total plate count of various semi‐conserved fishery products. While promoting discrete colony formation of Bacillus spp., PEDA provided good bacterial recovery. PEDA is recommended for isolation or enumeration of bacteria in food dominated by swarming Bacillus spp.