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Hydrolytic enzyme(s) production in Micrococcus roseus growing on different cellulosic substrates
Author(s) -
Paul Jaishree,
Varma A.K.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01386.x
Subject(s) - cellulosic ethanol , new delhi , micrococcus , library science , biology , history , bacteria , computer science , biochemistry , cellulose , archaeology , genetics , metropolitan area
Micrococcus roseus (G12) isolated from higher termite Odontotermes obesus gut exhibited cellulose digesting properties. A lignocellulosic substrate, rice husk induced endoglucanase, β‐glucosidase, β‐xylanase and β‐xylosidase production. Besides rice husk, CMC also induced endoglucanase production. β‐Glucosidase activity was quite pronounced when rice husk was supplemented with CMC or cellobiose. Both β‐xylanase and β‐xylosidase activities could be induced by xylan as well as xylobiose, whereas CMC induced partial activity. Endoglucanase and β‐xylanase enzymes were secreted into the culture medium, whereas β‐glucosidase and β‐xylosidase activities were intracellular in nature. Enzyme production was subject to end product inhibition. The extracellular enzyme(s) possessed the potential to saccharify rice husk, xylan and CMC to reducing sugars.