Premium
Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection
Author(s) -
Mansfield Lucielle P.,
Forsythe S. J.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01375.x
Subject(s) - salmonella , tetrathionate , food science , salmonella enteritidis , rappaport , immunomagnetic separation , biology , brilliant green , raw milk , food microbiology , skimmed milk , microbiology and biotechnology , chemistry , bacteria , theology , organic chemistry , philosophy , genetics
One hundred and twenty foodstuffs were tested for the enrichment of Salmonella species by immunoseparation. The foodstuffs covered six groups: raw chicken, prawns, skimmed milk powder, herbs and spices, cocoa powder and animal feed. Half of the food samples were spiked with one Salmonella species: Salm. ealing, Salm. enteritidis, Salm. give, Salm. typhimurium or Salm. virchow . Comparison of Salmonella recovery with standard methods (selenite cystine broth, tetrathionate broth and Rappaport‐Vassiliadis broth) was carried out. Immunoseparation gave similar numbers of true positives to the standard enrichment methods in a short time period. Only immunoseparation isolated Salmonella species from spiked garlic granules demonstrating the possible recovery of sublethally injured cells.