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Membrane potential‐dependent glycine uptake into vesicles of Brochothrix thermosphacta
Author(s) -
Singh Satya P.,
Rogers P. J.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01372.x
Subject(s) - ionophore , glycine , vesicle , membrane , chemistry , hydrazone , biochemistry , cyanide , biophysics , stereochemistry , biology , organic chemistry , amino acid
Glycine uptake in right‐side‐out membrane vesicles of Brochothrix thermosphacta was investigated. NADH and D‐lactate‐based potential (‐50 mV) supported glycine uptake up to a fivefold accumulation ratio. The uptake was sensitive to the addition of carbonyl cyanide m ‐chloro‐phenyl hydrazone (CCCP), an ionophore.