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A rapid luminescent‐phage based MPN method for the enumeration of Salmonella typhimurium in environmental samples
Author(s) -
Turpin P. E.,
Maycroft K. A.,
Bedford J.,
Rowlands C. L.,
Wellington E. M. H.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb01364.x
Subject(s) - library science , salmonella , enumeration , biology , computer science , mathematics , combinatorics , genetics , bacteria
Materials such as soils, waters, sewage sludges and foods can contain low numbers of salmonellas. A most‐probable‐number (MPN) method that utilized a bioluminescent‐bacteriophage is described that allowed the specific determination of as few as one Salmonella typhimurium cell/100 ml of material within 24 h. The method was developed with soil, lake water and sewage sludge inoculated with Salm. typhimurium and had an efficiency of 100% when tested against a traditional MPN method. The protocol is rapid, sensitive, inexpensive, has a low operator time compared to the traditional MPN method, allows for the repair of injured cells and is amenable to automation.