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Quantitative assessment of sterilization efficiency using lyophilized calcium alginate biological indicators
Author(s) -
Hanlon G. W.,
Hodges N. A.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb00387.x
Subject(s) - sterilization (economics) , bacillus subtilis , spore , bacillales , calcium alginate , geobacillus stearothermophilus , calcium , dry heat , chromatography , chemistry , bacillaceae , bacteria , biology , microbiology and biotechnology , materials science , organic chemistry , composite material , monetary economics , economics , foreign exchange market , genetics , foreign exchange , thermophile
Biological indicators were prepared using either Bacillus stearothermophilus or Bacillus subtilis incorporated into lyophilized calcium alginate beads. These were subjected to a variety of moist heat and dry heat treatments as appropriate and then dissolved in buffer to allow enumeration of survivors. The utility of these spore carriers as biological indicators for the quantitative assessment of heat sterilization processes is discussed.