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A rapid method of evaluating permeabilizing activity against Pseudomonas aeruginosa
Author(s) -
Ayres Helen,
Furr J. R.,
Russell A. D.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb00381.x
Subject(s) - welsh , pharmacy , library science , history , medicine , family medicine , archaeology , computer science
A rapid (< 30 min) spectrophotometric method has been used to assess the ability of chemical agents to permeabilize the outer membrane of Pseudomonas aeruginosa. When permeabilized, the cells are rendered readily sensitive to lysozyme, an enzyme that is normally excluded from the peptidoglycan of this organism. In addition to ethylenediamine tetraacetic acid, substances showing permeabilizing activity were citric, isocitric, malic and gluconic acids (but not gluconic acid lactone) and sodium polyphosphate.