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A simple most probable number technique for the sensitive recovery of a genetically‐modified Pseudomonas aureofaciens from soil
Author(s) -
Leij F. A. A. M. de,
Bailey M. J.,
Whipps J. M.,
Lynch J. M.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb00364.x
Subject(s) - pseudomonas , kanamycin , bacteria , pseudomonadaceae , biology , most probable number , pseudomonadales , microbiology and biotechnology , enrichment culture , strain (injury) , soil microbiology , genetics , anatomy
A strain of Pseudomonas aureofaciens (SBW25EeZY‐6KX) that was chromosomally marked with a lacZY and a km r ‐xylE cassette could be recovered from non‐sterile soil by a selective Pseudomonas enrichment broth amended with 100 ppm kanamycin and 50 ppm X‐gal in an MPN (most probable number) assay. The assay was sensitive and reliable, allowing detection of as few as one recombinant cell in a 1% (w v) soil suspension. The soil used contained a large ( ca 5% of total culturable bacteria) background of indigenous bacteria that were either able to utilize lactose, were resistant to kanamycin, or both.