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Rapid extraction of high purity chromosomal DNA from Serratia marcescens
Author(s) -
Alonso R.,
Nicholson P. S.,
Pitt T. L.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb00348.x
Subject(s) - serratia marcescens , restriction enzyme , dna , enzyme , biology , endonuclease , microbiology and biotechnology , yield (engineering) , biochemistry , enterobacteriaceae , thiocyanate , dna extraction , chemistry , chromatography , escherichia coli , polymerase chain reaction , materials science , metallurgy , gene
Rapid non‐specific degradation of Serratia marcescens DNA extracted with guanidium thiocyanate, occurred within 10 min of incubation with restriction endo‐nuclease enzymes. The described modified method based on chemical and enzymatic deproteinization produced preparations of Ser. marcescens DNA of high yield and quality which did not autodegrade when incubated with restriction endonucleases.