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Thermal denaturation and loss of viability in Escherichia coli and Bacillus stearothermophilus
Author(s) -
Mackey B. M.,
Miles C. A.,
Seymour D. A.,
Parsons S. E.
Publication year - 1993
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1993.tb00341.x
Subject(s) - escherichia coli , denaturation (fissile materials) , differential scanning calorimetry , bacillales , bacillus (shape) , biology , bacillaceae , bacteria , chemistry , microbiology and biotechnology , biochemistry , nuclear chemistry , bacillus subtilis , thermodynamics , genetics , gene , physics
When Escherichia coli was heated at 10°C/min in a differential scanning calorimeter, the onset of irreversible thermal denaturation occurred at 51°C, about 5°C above the maximum growth temperature. The temperature at which death rate was maximal (63°C) coincided with the thermogram peak caused by denaturation of the 30S ribosomal subunit. The maximum death rate in vegetative cells of Bacillus stearothermophilus occurred at the higher temperature of 71°C which also coincided with the leading edge of the main thermogram peak.