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Increased susceptibility of injured spores of Bacillus subtilis to cationic and other stressing agents
Author(s) -
Williams Nicola D.,
Russell A.D.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00776.x
Subject(s) - spore , bacillus subtilis , microbiology and biotechnology , agar , polymyxin , chemistry , sodium , biocide , sodium hydroxide , bacteria , biology , antibiotics , genetics , organic chemistry
Sublethally heated spores of Bacillus subtilis NCTC 8236 were susceptible to posttreatment concentrations in agar of polymyxin B sulphate, sodium hydroxide, cetylpyridinium chloride and sodium lauryl sulphate that did not prevent colony formation to untreated spores. The non‐ionic surfactants polysorbates 20 and 80 were not inhibitory when used at high concentrations against both heated and unheated spores. The method has been developed for detecting sublethal injury in biocide‐exposed spores, since iodine‐treated spores became highly susceptible to polymyxin contained in recovery agar.