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Cross‐reactivity of bacteriocins from lactic acid bacteria and lantibiotics in a nisin bioassay and ELISA
Author(s) -
Falahee M.B.,
Adams M.R.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00766.x
Subject(s) - nisin , lantibiotics , bacteriocin , bioassay , bacteria , microbiology and biotechnology , lactococcus lactis , biology , cross reactivity , lactic acid , chemistry , biochemistry , cross reactions , antimicrobial , antibody , immunology , genetics
A number of bacteriocins from lactic acid bacteria and lantibiotics were tested for cross‐reactivity in a nisin ELISA and bioassay. The bacteriocins showed no cross‐reactivity, reflecting their structural dissimilarity from nisin. The lantibiotic subtilin which shares many common structural features with nisin, showed a high cross‐reactivity in both the ELISA and the bioassay suggesting possible modifications to nisin to enhance its activity. Gallidermin did not cross react in the ELISA but did produce a zone of inhibition in the less specific bioassay. Other lantibiotics tested did not react in either assay.