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Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification
Author(s) -
Hiraishi A.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00765.x
Subject(s) - polymerase chain reaction , 16s ribosomal rna , biology , dna sequencer , dna , microbiology and biotechnology , dna sequencing , ribosomal dna , gene , polymerase , bacteria , multiple displacement amplification , genetics , dna extraction , phylogenetics
The 16S rRNA gene from various bacterial cultures was amplified by the poly‐merase chain reaction without DNA purification, and sequenced directly by using a laser fluorescent DNA sequencer and Tth polymerase with a cycle sequencing protocol. The described procedures provide almost complete 16S rDNA sequence data within a couple of days and facilitate systematic studies.