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Plasmid‐encoded resistance to tetracycline in Staphylococcus haemolyticus
Author(s) -
Schwarz S.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00748.x
Subject(s) - plasmid , staphylococcus haemolyticus , biology , tetracycline , microbiology and biotechnology , transformation (genetics) , nuclease , restriction map , staphylococcus , genetics , gene , bacteria , staphylococcus aureus , antibiotics
A small 4.35 kb plasmid, designated pSTS4, was isolated from a multiresistant Staphylococcus haemolyticus culture. It conferred resistance to tetracycline as shown by protoplast transformation. pSTS4 was further characterized by restriction endo‐nuclease analyses and a preliminary restriction map constructed. It shared some structural similarities with previously described small Tc R plasmids from other staphylococcal species of human and animal origin. pSTS4 encoded two proteins of approximately 37 kDa and 52 kDa as revealed by combined in vitro transcription/translation assays.